Insight into Membrane Stability and Physiological Responses of Selected Salt-Tolerant and Salt-Sensitive Cell Lines of Troyer Citrange (Citrus sinensis [L.] x Citrus trifoliata [L.] Raf.) under Salt Stress

The aim of this study was to evaluate the membrane integrity and some physiological responses rootstock citrus calli under exposure different concentrations NaCl. Selected salt-tolerant cell lines were compared with salt-sensitive Troyer’s citrange (Citrus sinensis [L.] x Citrus trifoliata Raf.) (TC) respect growth, water content, Na+, K+ Cl? ion content as well stability NaCl concentrations. results show that stressed sensitive have a consistently high efflux. values recorded for these are 3 6 times higher than those tolerant calli. Thus, only selected halotolerant able maintain their membranes salt stress conditions. In calli, always induces slowing down growth even from 4 g L?1, reduction in relative rate is 50% reaches more 90% three culture durations at 8 L?1 For lines, seems be slightly slowed until second month but becomes equal control third month, whether or At end 100% twice ones after months treatment After long-term culture, absorbed similar amounts Na+ lines. However, by 3rd accumulation dropped unselected continued increase (4-fold 12 control). Furthermore, both types (salt-sensitive salt-tolerant) resulted greater loss NaCl-sensitive uptake not affected decrease tissue less 25% From observation, it can concluded ability retain levels internal correlated.